Calibration – Read Calibrated Images

To read a calibrated image, from a directory that has a calibration file, check the “calibration” flag in either ReadStack or ReadImage. The Read module will look up the name of each file in the calibration file, get the mean value of the calibration target, and divide the incoming values by the calibration value, producing calibrated data on the fly.

The example below shows a PCA computation. The dialog window indicates that the “input” image is on the left, and the “ROI” image is from the top. Note the small window for analysis of the “ROI” (region of interest) image. Also note that both ReadStack modules have the “calibration” flag checked. Calibrating the data, before analyzing it with PCA, may give a better result.